Name: Aflatoxin A ELISA Kit
Brand: Biological test kits, ELISA (enzyme-linked immunosorbent assay) kits, chemical reagents-Shanghai IDEAL MEDICAL TECH Co. Ltd.
SKU: ADL-EL-PR00001
Availability: InStock
Rating: 5 (1 reviews)

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ELISA Kit

Researchuse ELISA kit

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Home > ELISA Kit > Researchuse ELISA kit > Food Safety Elisa Kit

Description

Experimental principle:

Coat the target antibody in a 96 well microplate to form a solid phase carrier. Add standard or specimen to each well, where the target is bound to the antibody attached to the solid phase carrier. Then add the microbialized target antibody. Wash the unbound biotin antibody, add HRP label and avidin, wash thoroughly again, and add TMB substrate for color development. TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. The depth of color is positively correlated with the target in the sample. Measure the absorbance (O.D. value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader and calculate the sample concentration.

Kit composition:

Test kit operation steps:

1. Take out the required Flat noodles from the aluminum foil bag after 20 min of room temperature balance, and seal the remaining Flat noodles with a self sealing bag and put it back at 4 ℃.

2. Set up standard wells and sample wells, and add 50 μ L of standard samples of different concentrations to each standard well.

3. Add 50 μ L of the sample to be tested into the sample well; Blank holes are not added.

4. Except for blank wells, add 100 μ L of horseradish peroxidase (HRP) labeled detection antibody to each well of the standard and sample wells, seal the reaction well with a sealing plate membrane, and incubate at 37 ℃ in a water bath or constant temperature box for 60 minutes.

5. Discard the liquid, pat dry on absorbent paper, and fill each well with washing solution (350 μ L), Let it stand for 1 minute, shake off the washing solution, pat dry on absorbent paper, and repeat the washing process 5 times (or use a washing machine to wash the board).

6. Add 50 μ L of substrate A and B to each well, and incubate at 37 ℃ in the dark for 15 minutes.

7. Add 50 μ L of termination solution to each well and measure the OD value of each well at a wavelength of 450nm within 15 minutes.

Self provided experimental equipment required for the experiment:

1. ELISA reader (450nm)

2. High precision sampler and nozzle: 0.5-10uL, 2-20uL, 20-200uL, 200-1000uL

3. 37 ℃ constant temperature box

4. Distilled water or deionized water

Experimental result calculation:

Draw standard curve: In an Excel worksheet, use the standard concentration as the horizontal axis and the corresponding OD value as the vertical axis to draw a linear regression curve of the standard. Calculate the concentration values of each sample according to the curve equation.

Test kit performance:
Please request detailed electronic instructions from customer service personnel!

Technical tip:

1.When mixing or redissolving protein solutions, try to avoid foaming as much as possible.

2.To avoid cross infection, it is necessary to replace the nozzle when configuring different concentration standards, loading samples, and adding different reagents. Additionally, please use different pipettes for different reagents.

3. During each incubation, please use the sealing glue correctly to ensure the accuracy of the results.

4. The mixed chromogenic substrate should be colorless before being loaded onto the plate. Please store it away from light; If the microplate changes from being searched to different depths of blue.

5. The order of terminating the liquid on the plate should be consistent with the order of the color developing substrate on the plate; After adding the termination solution, the color inside the hole changed from blue to yellow; If there is green inside the hole, it indicates that the liquid inside the hole is not mixed evenly; Please mix thoroughly.

Product expiration date: 6 months

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Aflatoxin A ELISA Kit

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