Name: 48T/96T Bovine Apolipoprotein A5 (apo-A5) ELISA Kit
Brand: Biological test kits, ELISA (enzyme-linked immunosorbent assay) kits, chemical reagents-Shanghai IDEAL MEDICAL TECH Co. Ltd.
SKU: ADL-EL-BV00195
Availability: InStock
Rating: 5 (1 reviews)

We use cookies to improve your online experience. By continuing browsing this website, we assume you agree our use of cookies.

English

日本語

Deutsch

Français

Español

Русский

Português

简体中文

Italiano

한국어

ไทย

Tiếng Việt

Home

Products

ELISA Kit

Researchuse ELISA kit

Human Elisa Kit Rat ELISA kit Mouse ELISA kit Bovine ELISA Kit Sheep ELISA kit Fish ELISA kits Agriculture of plant viruses, bacteria, fungi, plant hormones elisa kit Microbial ELISA kits Nematode-Insect ELISA Kit Other species ELISA kits Pig Elisa Kit Poultry Elisa Kit Horse Elisa Kit Food Safety Elisa Kit Chicken Elisa Kit Duck Elisa Kit Canine Elisa Kit Cat Elisa Kit Cavy Elisa Kit Rabbit Elisa Kit Monkey Elisa Kit

IVD

cytokine Liver fibrosis myocardial infarction endocrine Autoimmunity Tumor markers Well-Bear and Well-Rear Infectious Diseases Specific Proteins

Biochemical reagent kit

Biochemical Kit

amino acid protease Nitrogen metabolism starch Ions Tricarboxylic acid cycle Sugar metabolism Glycolysis Gluconeogenesis signal Fatty acid metabolism Soil Other

Biochemical Reagents

Colloidal gold rapid detection reagent kit

Animal

Canine diseases Cat diseases Bovine/sheep diseases Swine diseases Avian diseases

Human

Women's Healthcare Rapid Test Kits TORCH Rapid Test Kits Hormone Rapid Test Kits Hepatitis Rapid Test Kits Gastrointestinal diseases Respiratory system diseases Sexually transmitted diseases Tropical virus Cardiac Markers Tumor markers Single Common Drugs Of Abuses (DOAs) Urine Rapid Tests Drugs Of Abuses (DOAs) Urine Rapid Tests-Single New Drug Urine Rapid Test Kits Drugs Of Abuses (DOAs) Urine Rapid Tests-Multi-Drug Urine Rapid Test Kits Drugs Of Abuse (DOA) Saliva Rapid Tests-Single Drug Saliva Rapid Test Kits Drugs Of Abuses (DOAs) Saliva Rapid Tests-Multi-Drug Saliva Rapid Test Kits Others

PCR reagent kit/PCR reagent and kit

DNA product purification series/DNA extraction reagent and kit RNA product purification series Preservation liquid series/Sample preservation eagent Protein Research Series/Proten Nucleic acid amplification-electrophoresis series Genomic Research Series Nucleic acid testing series others

Culture Medium

Laboratory equipment

ELISA reader Centrifuges Elisa washer spectrometer Microplate Pipette other

Emergency series

others respirator First aid kit emergency stretcher wheelchair auto hematology analyzer Stethoscope electrocardiograph Medical adhesive tape emergency supply Tactical backpack hospital bed

Blood culture instrument

News

Company News

Industry News

About Us

Feedback

Português

Home > ELISA Kit > Researchuse ELISA kit > Bovine ELISA Kit

48T/96T Bovine Apolipoprotein A5 (apo-A5) ELISA Kit

Description Common Elisa types Quality control Introduction to techology

Description

Name	Bovine Apolipoprotein A5 (apo-A5) ELISA Kit
specification	96T
Item No.	HB017-Bv
species	Cattle
Sample type	Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant, etc.
scope of test	See instructions for details
Sensitivity	See instructions for details
Reaction time	1-5h
Sample volume	10ul
Detection wavelength	450nm
Delivery time	3-5 days
Manual	Consult our salesperson to obtain

Precision

The precision is expressed by the coefficient of variation CV of the measured value of the sample. CV (%) = SD/mean×100
Intra-batch difference: Take the same batch of kits for quantitative detection of low, medium, and high-value fixed value samples. Each sample is measured 20 times continuously, and the average value and SD value of samples of different concentrations are calculated.
Inter-batch difference: select 3 different batches of kits to quantitatively determine the low, medium, and high-value fixed value samples. Use the same kit for each sample to repeat the measurement 10 times, and calculate the average value and SD of samples with different concentrations. value.
Intra-run difference: CV<5.8%
Difference between batches: CV<9.2%

Kit composition

Serial number	Name	specification
1	Wash solution(30X)	20ml×1 bottle
2	HRP-Conjugate reagent	6ml×1 bottle
3	Microelisa stripplate	12 holes×8 strips
4	Sample diluent	6ml×1 bottle
5	Chromogen Solution A	6ml×1 bottle
6	Chromogen Solution B	6ml×1/bottle
7	Stop solution	6ml×1 bottle
8	Standard	0.5ml×1 bottle
9	Standard diluent	1.5ml×1 bottle
10	Product Description	1 serving
11	Sealing plate membrane	2 sheets
12	Sealing bag	1 piece

Note: Please check whether the label and quantity of the reagents in the kit are consistent with the table before use.

Materials and consumables needed but not provided
1. Microplate reader (it is recommended to warm up the instrument before use)
2. Precise pipettes, tips, and sample tanks
3. Distilled or deionized water
4. Bottle washing or automatic plate washing machine
5. 37℃ water bath or thermostat
6. EP tube
7. Powder-free disposable latex gloves

Storage conditions and validity period
1. Store at 2-8℃, do not freeze, the validity period is 6 months.
2. After opening and use, put the coated microplate into a ziplock bag with desiccant, seal the ziplock bag, and put all reagents back into the refrigerator at 2-8°C.

Common Elisa types

ELISA can be used to detect both antigens and antibodies.
Samples to be tested usually include serum, plasma, cell supernatant, tissue homogenate, etc.
Here are three necessary reagents in this assay method:

1. Antigen or antibody on solid phase, ie "immunosorbent"

2. Enzyme-labeled antibodies or antibodies, i.e. "conjugates";

3. Enzyme reaction substrate.

According to the source of the reagent, the nature of the sample, and the relevant conditions of the detection, a variety of detection methods

with different purposes can be designed.

Quality control

Strict R&D and verification system to ensure product performance

1.carefully screened pairings

In the double-antibody sandwich method, capture antibodies and detection

antibodies must pass strict screening, and then carry out scientific

concentration adjustment.

2. reliable specificity

By carefully selecting antibody pairs and optimizing buffers, matrix

effects are eliminated and only target analytes are recognized.

3. a variety of experimental principles

The double-antibody sandwich method, indirect method, and competition

method are designed to meet the detection of proxies with different

molecular weights.

4.The sample type has been verified

It can detect various samples such as serum, plasma,

culture supernatant, tissue, etc.

5. Good stability

6. Strict verification tests, including but not limited to the following

a.Intra-panel and inter-panel accuracy
b.Authenticated samples, such as serum, plasma, cell supernatant and other natural sample analysis
c.Blood component interference experiment
d.Linear
e.Recovery rate
f.Reagents and components are stable

These verification processes are reviewed by professional quality assurance personnel to ensure that the test results meet the guidelines and conform to the established technical specifications.

Introduction to techology

ELISA stands for Enzyme-Linked,Immunosorbent Assay, also often referred to as Enzyme Immunoassay (EIA). ELISA, like other types of immunoassays, relies on antibodies and utilizes highly specific antibody-antigen interactions to detect the target antigen. ELISA is a classic experiment in immunology. Due to its high sensitivity, strong specificity and good repeatability, it has developed rapidly since it came out in the 1970s and is now widely used in many fields ,such as immune diagnosis, detection and biology.

ELISA principle (see the picture on the right)

Antigen-antibody immobilization

Known antigen/antibody binds to the bottom of the microtiter plate and maintains its immunological activity

Enzyme marking

Label antigen/antibody and maintain its immunocompetence and enzymatic activity

Substrate coloration

The substrate is catalyzed by the enzyme into a colored product, and the amount of the product is directly related to the amount of the substance to be tested in the sample, which can be
qualitatively or quantitatively analyzed according to the depth of the color

Signal amplification

Due to the high catalytic efficiency of the enzyme, the results of the immune reaction are indirectly amplified, so that the assay method can achieve high sensitivity

Send your message to us

First Name

* Email

Country

please select your country

Afghanistan
Aland Islands
Albania
Algeria
American Samoa
Andorra
Angola
Anguilla
Antigua and Barbuda
Argentina
Armenia
Aruba
Australia
Austria
Azerbaijan
Bahamas
Bahrain
Bangladesh
Barbados
Belarus
Belgium
Belize
Benin
Bermuda
Bhutan
Bolivia
Bosnia and Herzegovina
Botswana
Bouvet Island
Brazil
British Indian Ocean Territory
British Virgin Islands
Brunei Darussalam
Bulgaria
Burkina Faso
Burundi
Cambodia
Cameroon
Canada
Cape Verde
Caribbean Netherlands
Cayman Islands
Central African Republic
Chad
Chile
China
Christmas Island
Cocos Islands
Colombia
Comoros
Congo
Cook Islands
Costa Rica
Cote D'ivoire
Cuba
Curaçao
Cyprus
Czech Republic
Democratic People's Republic of Korea
Democratic Republic of the Congo
Denmark
Djibouti
Dominica
East Timor
Ecuador
Egypt
El Salvador
Equatorial Guinea
Eritrea
Estonia
Ethiopia
Falkland Islands
Faroe Islands
Fiji
Finland
France
French Guiana
French Polynesia
French Southern Territories
Gabon
Gambia
Georgia
Germany
Ghana
Gibraltar
Greece
Greenland
Grenada
Guadeloupe
Guam
Guatemala
Guernsey
Guinea
Guinea-Bissau
Guyana
Haiti
Heard Island and Mcdonald Islands
Honduras
Hong Kong, China
Hungary
Iceland
India
Indonesia
Iran
Iraq
Ireland
Isle of Man
Israel
Italy
Jamaica
Japan
Jordan
Kazakhstan
Kenya
Kiribati
Korea
Kosovo
Kuwait
Kyrgyzstan
Laos
Latvia
Lebanon
Lesotho
Liberia
Liechtenstein
Lithuania
Luxembourg
Macau, China
Macedonia
Madagascar
Malawi
Malaysia
Maldives
Mali
Malta
Marshall Islands
Martinique
Mauritania
Mauritius
Mayotte
Mexico
Micronesia
Moldova
Monaco
Mongolia
Montenegro
Montserrat
Morocco
Mozambique
Myanmar
Namibia
Nauru
Nepal
Netherlands
Netherlands Antilles
New Caledonia
New Zealand
Nicaragua
Niger
Nigeria
Niue
Norfolk Island
Northern Mariana Islands
Norway
Oman
Pakistan
Palau
Palestine
Panama
Papua New Guinea
Paraguay
Peru
Philippines
Pitcairn Islands
Poland
Portugal
Puerto Rico
Qatar
Reunion
Romania
Russia
Rwanda
Saint Barthélemy
Saint Helena
Saint Kitts and Nevis
Saint Lucia
Saint Martin
Saint Pierre and Miquelon
Saint Vincent and the Grenadines
San Marino
Sao Tome and Principe
Saudi Arabia
Senegal
Serbia
Seychelles
Sierra Leone
Singapore
Sint Maarten
Slovakia
Slovenia
Solomon Islands
Somalia
South Africa
South Georgia and The South Sandwich Islands
Spain
Sri Lanka
State of Libya
Sudan
Suriname
Svalbard and Jan Mayen
Swaziland
Sweden
Switzerland
Syrian Arab Republic
TaiWan, China
Tajikistan
Tanzania
Thailand
The Republic of Croatia
Togo
Tokelau
Tonga
Trinidad and Tobago
Tunisia
Turkey
Turkmenistan
Turks and Caicos Islands
Tuvalu
Uganda
Ukraine
United Arab Emirates
United Kingdom
United States
United States Minor Outlying Islands
Uruguay
US Virgin Islands
Uzbekistan
Vanuatu
Vatican City State
Venezuela
Vietnam
Wallis and Futuna Islands
Western Sahara
Western Samoa
Yemen
Zambia
Zimbabwe

* Phone

* Message

Our strong technical research and development team ensures that our pre-sales and post-sales are timely, we provide free guidance throughout the whole process, and if necessary, we can come to your home pre-sales and post-sales.

Products

About Us

Add: Liqin Building, 1885 Duhui Road, Minhang District, Shanghai, China

TEL: +86 18321012158

E-mail: Info@idealmedicaltech.com

Anna

Leave a message

Language

Language

48T/96T Bovine Apolipoprotein A5 (apo-A5) ELISA Kit

Size: