Name: Goat serotonin (5-HT) ELISA Kit
Brand: Biological test kits, ELISA (enzyme-linked immunosorbent assay) kits, chemical reagents-Shanghai IDEAL MEDICAL TECH Co. Ltd.
SKU: ADL-EL-SP00168
Availability: InStock
Rating: 5 (1 reviews)

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Researchuse ELISA kit

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Home > ELISA Kit > Researchuse ELISA kit > Sheep ELISA kit

Description

Name	Goat serotonin (5-HT) ELISA Kit
Specifications	96T
article number	HB127-Gt
Species	Goat
Sample type	serum, plasma, tissue homogenate,cell lysate, cell Culture supernatant etc.
Detection range	See the manual for details
Sensitivity	See the manual for details
Response time	1-5h
Sample volume	10ul
Detection wavelength	450nm
Delivery date	3-5 days
Manual	Consult our salesperson for obtaining

Precision
The precision is expressed by the coefficient of variation CV of the measured value of the sample. CV (%) = SD/mean×100
Intra-batch difference: Take the same batch of kits for quantitative detection of low, medium, and high value fixed value samples. Each sample is measured 20 times continuously, and the average value and SD value of samples of different concentrations are calculated.
Inter-batch difference: select 3 different batches of kits to quantitatively determine the low, medium, and high-value fixed value samples. Use the same kit for each sample to repeat the measurement 10 times, and calculate the average value and SD of samples with different concentrations. value.
Intra-run difference: CV<4.7%
Difference between batches: CV<7.7%
Recovery rate
The target protein of known concentration was added to 5 different samples, and the recovery experiment was performed to obtain the recovery range and average recovery rate.

Sample Type	Range (%)	Average Recovery (%)
Tissue Lysates (n=5)	94-108	99
Serum (n=5)	93-107	96
EDTA plasma (n=5)	86-106	94
Cell culture media (n=5)	82-104	93

Linear
Add the target protein of known concentration to 5 samples respectively, do recovery experiment, get the range of recovery rate and average recovery rate. Dilute 5 samples by 2 times, 4 times, 8 times, and 16 times respectively for recovery experiments to obtain the recovery range and average recovery rate.

Sample	1:2	1：4	1:8	1:16
Tissue Lysates (n=5)	92-105%	84-99%	90-102%	80-93%
Serum (n=5)	81-101%	89-99%	86-98%	83-101%
EDTA plasma (n=5)	85-97%	82-101%	83-96%	79-91%
Cell culture media (n=5)	83-105%	89-112%	81-106%	82-110%

Specificity
This kit recognizes natural and recombinant Goat serotonin (5-HT) , and has no obvious cross-reactivity with other similar substances. Due to the limitation of technology and sample source, it is impossible to complete the cross-reaction test of all related or similar substances. Therefore, this kit may have cross-reaction with other untested substances.

Stability
It has been determined that the kit must be stored at the recommended temperature within the validity period, and the activity reduction rate is less than 5%. In order to reduce the influence of external factors on the detection value before and after the destruction of the kit, the environmental conditions of the laboratory should be kept as consistent as possible, especially the temperature, humidity and incubation conditions in the laboratory. Secondly, the operation by the same experimenter can reduce human error.

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Goat serotonin (5-HT) ELISA Kit

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