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Human leptin(LEP)Elisa kit

Item No.: ADL-EL-HM00080
English name: Human leptin(LEP)Elisa kit
Adapted species: human
  • Specifications:
    48T
    96T
Description
Description
Name: Human leptin(LEP)Elisa kit
cas: ADL-EL-HM00080
oTHER NAME: Leptin ELISA Kit, LEP ELISA Kit, LEPD ELISA Kit, OBS ELISA Kit, Obesity Homolog ELISA Kit
taxonomy: Human
sIZE: 48T/96T
STORAGE:  2-8℃,6months
APPLICATION:

Quantitative in vitro determination of leptin (LEP)  concentrations in plasma, tissue homogenates and other biological fluids.

Detection Methods: Double antibody sandwich method
Detection range: 31.25-2000pg/ml
sensitivity: < 18.75pg/ml
standard curve:
recovery rate:

A certain amount of leptin (LEP) was mixed into the samples listed in the table below and the recovery was calculated by comparing the measured values with the expected amount of leptin (LEP) in the samples.

  Range(%) Average value(%)
plasma(n=5) 93-104 98
EDTA plasma(n=5) 86-104 96
heparin plasma(n=5) 93-101 97
linearity:

 The linearity of the kit was determined by assaying samples mixed with appropriate concentrations of leptin (LEP) and their serial dilutions, and the linearity was expressed as a percentage of the assayed concentration relative to the expectation.

Sample 1:2 1:4 1:8
plasma(n=5) 88-103% 91-102% 85-97%
EDTA plasma(n=5) 85-96% 85-99% 82-100%
heparin plasma(n=5) 80-99% 87-94% 82-94%
coefficient of variation(%):

Intra-Lot Differences: CV<8%

Inter-Lot Differences: CV<10%

caveat:

For scientific use only

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