Name: NO Content Assay Kit
Determination method: trace method
Specification: 100T/96S
Storage conditions: low temperature and light protection
Validity: 6 months
Note: Choose 2-3 samples with large expected differences for pre-calibration before formal determination.
Measurement significance:
NO (Nitric Oxide, NO) is widely distributed in organisms in the nervous, circulatory, respiratory, digestive, urogenital and other systems, especially more abundant in the nervous tissue. It acts as an intercellular and intracellular information substance and plays the role of signal transmission. It is a new type of biological messenger molecule, which plays an important role in the physiological and pathological processes of the organism.
Principle of measurement:
NO is very easy to oxidise in the body or in aqueous solution to generate NO2-, under acidic conditions, NO2- and diazonium salt sulfonate amine generate diazo compounds, which further couples with naphthalene-based vinyl diamine, and the product has a characteristic absorption peak at 550 nm, and the absorbance value is measured, so that the NO content can be calculated.
Provide your own lab supplies and apparatus:
Balance, mortar or homogeniser, visible spectrophotometer/enzymometer, micro quartz cuvette/96-well plate, distilled water.

