Name: Plant anthocyanin (anth) ELISA Kit
Brand: Biological test kits, ELISA (enzyme-linked immunosorbent assay) kits, chemical reagents-Shanghai IDEAL MEDICAL TECH Co. Ltd.
SKU: ADL-EL-PT00040
Availability: InStock
Rating: 5 (1 reviews)

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ELISA Kit

Researchuse ELISA kit

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Home > ELISA Kit > Researchuse ELISA kit > Agriculture of plant viru...

Description Common Elisa types Quality control Introduction to techology

Description

Name	Plant anthocyanin (anth) ELISA Kit
Specifications	96T
article number	HB196-Pt
Species	Plant
Sample type	serum, plasma, tissue homogenate,cell lysate, cell Culture supernatant etc.
Detection range	See the manual for details
Sensitivity	See the manual for details
Response time	1-5h
Sample volume	10ul
Detection wavelength	450nm
Delivery date	3-5 days
Manual	Consult our salesperson for obtaining

Precision
The precision is expressed by the coefficient of variation CV of the measured value of the sample. CV (%) = SD/mean×100
Intra-batch difference: Take the same batch of kits for quantitative detection of low, medium, and high value fixed value samples. Each sample is measured 20 times continuously, and the average value and SD value of samples of different concentrations are calculated.
Inter-batch difference: select 3 different batches of kits to quantitatively determine the low, medium, and high-value fixed value samples. Use the same kit for each sample to repeat the measurement 10 times, and calculate the average value and SD of samples with different concentrations. value6
Intra-run difference: CV<4.3%
Difference between batches: CV<7.6%
Recovery rate
The target protein of known concentration was added to 5 different samples, and the recovery experiment was performed to obtain the recovery range and average recovery rate.

Sample Type	Range (%)	Average Recovery (%)
Tissue Lysates (n=5)	91-108	97
Serum (n=5)	83-106	92
EDTA plasma (n=5)	85-105	94
Cell culture media (n=5)	93-107	96

Linear
Add the target protein of known concentration to 5 samples respectively, do recovery experiment, get the range of recovery rate and average recovery rate. Dilute 5 samples by 2 times, 4 times, 8 times, and 16 times respectively for recovery experiments to obtain the recovery range and average recovery rate.

Sample	1:2	1：4	1:8	1:16
Tissue Lysates (n=5)	92-105%	84-99%	90-102%	80-93%
Serum (n=5)	81-101%	89-99%	86-98%	83-101%
EDTA plasma (n=5)	85-97%	82-101%	83-96%	79-91%
Cell culture media (n=5)	83-105%	89-112%	81-106%	82-110%

Specificity
This kit recognizes natural and recombinant Plant anthocyanin (anth) , and has no obvious cross-reactivity with other similar substances. Due to the limitation of technology and sample source, it is impossible to complete the cross-reaction test of all related or similar substances. Therefore, this kit may have cross-reaction with other untested substances.

Stability
It has been determined that the kit must be stored at the recommended temperature within the validity period, and the activity reduction rate is less than 5%. In order to reduce the influence of external factors on the detection value before and after the destruction of the kit, the environmental conditions of the laboratory should be kept as consistent as possible, especially the temperature, humidity and incubation conditions in the laboratory. Secondly, the operation by the same experimenter can reduce human error.

Kit composition

Serial number	English name	specification
1	Wash solution(30X)	20ml×1 bottle
2	HRP-Conjugate reagent	6ml×1 bottle
3	Microelisa stripplate	12 holes×8 strips
4	Sample diluent	6ml×1 bottle
5	Chromogen Solution A	6ml×1 bottle
6	Chromogen Solution B	6ml×1/bottle
7	Stop solution	6ml×1 bottle
8	Standard	0.5ml×1 bottle
9	Standard diluent	1.5ml×1 bottle
10	Product Description	1 serving
11	Sealing plate membrane	2 sheets
12	Sealing bag	1 piece

Note: Please check whether the label and quantity of the reagents in the kit are consistent with the table before use.

1. Microplate reader (it is recommended to warm up the instrument before use)
2. Precise pipettes, tips, and sample tanks
3. Distilled or deionized water
4. Bottle washing or automatic plate washing machine
5. 37℃ water bath or thermostat
6. EP tube
7. Powder-free disposable latex gloves

Storage conditions and validity period
1. Store at 2-8℃, do not freeze, the validity period is 6 months.
2. After opening and use, put the coated microplate into a ziplock bag with desiccant, seal the ziplock bag, and put all reagents back into the refrigerator at 2-8°C.

Common Elisa types

ELISA can be used to detect both antigens and antibodies.
Samples to be tested usually include serum, plasma, cell supernatant, tissue homogenate, etc.
Here are three necessary reagents in this assay method:

1. Antigen or antibody on solid phase, ie "immunosorbent"

2. Enzyme-labeled antibodies or antibodies, i.e. "conjugates";

3. Enzyme reaction substrate.

According to the source of the reagent, the nature of the sample, and the relevant conditions of the detection, a variety of detection methods

with different purposes can be designed.

Quality control

Strict R&D and verification system to ensure product performance

1.carefully screened pairings

In the double-antibody sandwich method, capture antibodies and detection

antibodies must pass strict screening, and then carry out scientific

concentration adjustment.

2. reliable specificity

By carefully selecting antibody pairs and optimizing buffers, matrix

effects are eliminated and only target analytes are recognized.

3. a variety of experimental principles

The double-antibody sandwich method, indirect method, and competition

method are designed to meet the detection of proxies with different

molecular weights.

4.The sample type has been verified

It can detect various samples such as serum, plasma,

culture supernatant, tissue, etc.

5. Good stability

6. Strict verification tests, including but not limited to the following

a.Intra-panel and inter-panel accuracy
b.Authenticated samples, such as serum, plasma, cell supernatant and other natural sample analysis
c.Blood component interference experiment
d.Linear
e.Recovery rate
f.Reagents and components are stable

These verification processes are reviewed by professional quality assurance personnel to ensure that the test results meet the guidelines and conform to the established technical specifications.

Introduction to techology

ELISA stands for Enzyme-Linked,Immunosorbent Assay, also often referred to as Enzyme Immunoassay (EIA). ELISA, like other types of immunoassays, relies on antibodies and utilizes highly specific antibody-antigen interactions to detect the target antigen. ELISA is a classic experiment in immunology. Due to its high sensitivity, strong specificity and good repeatability, it has developed rapidly since it came out in the 1970s and is now widely used in many fields ,such as immune diagnosis, detection and biology.

ELISA principle (see the picture on the right)

Antigen-antibody immobilization

Known antigen/antibody binds to the bottom of the microtiter plate and maintains its immunological activity

Enzyme marking

Label antigen/antibody and maintain its immunocompetence and enzymatic activity

Substrate coloration

The substrate is catalyzed by the enzyme into a colored product, and the amount of the product is directly related to the amount of the substance to be tested in the sample, which can be
qualitatively or quantitatively analyzed according to the depth of the color

Signal amplification

Due to the high catalytic efficiency of the enzyme, the results of the immune reaction are indirectly amplified, so that the assay method can achieve high sensitivity

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Plant anthocyanin (anth) ELISA Kit

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