Name: Rat Calcitonin（CT）ELISA KIT
Brand: Biological test kits, ELISA (enzyme-linked immunosorbent assay) kits, chemical reagents-Shanghai IDEAL MEDICAL TECH Co. Ltd.
SKU: ADL01977R1
Availability: InStock
Rating: 5 (1 reviews)

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ELISA Kit

Researchuse ELISA kit

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Home > ELISA Kit > Researchuse ELISA kit > Rat ELISA kit

Description

Rat Calcitonin（CT）ELISA KIT
Specifications	48T/96T
article number	ADL01977R1
Species	rat
Sample type	serum, plasma, tissue homogenate,cell lysate, cell Culture supernatant etc.
Detection range	See the manual for details
Sensitivity	See the manual for details
Response time	1-5h
Sample volume	100 μL
Detection wavelength	450nm
Delivery date	3-5 days
Manual	Consult our salesperson for obtaining

Principle of the assay

The kit assay Rat CT level in the sample，use Purified Rat CT antibody to coat microtiter plate wells, make solid-phase antibody, then add CT to wells, Combined CT antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat CT in the samples is then determined by comparing the O.D. of the samples to the standard curve

Recovery rate
The target protein of known concentration was added to 5 different samples, and the recovery experiment was performed to obtain the recovery range and average recovery rate.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	94-110	102
EDTA plasma (n=5)	90-102	96
Cell culture media (n=5)	95-106	98

Linear
Add the target protein of known concentration to 5 samples respectively, do recovery experiment, get the range of recovery rate and average recovery rate. Dilute 5 samples by 2 times, 4 times, 8 times, and 16 times respectively for recovery experiments to obtain the recovery range and average recovery rate.

Sample	1:2	1：4	1:8	1:16
Serum (n=5)	99-108%	95-109%	89-103%	88-102%
EDTA plasma (n=5)	95-105%	90-100%	89-104%	98-108%
Cell culture media (n=5)	92-104%	97-105%	96-108%	92-105%

Specificity
This kit recognizes natural and Rat soluble leukocyte differentiation antigen 30 ligand (scd30l), and has no obvious cros-reactivity with other similar substances. Due to the limitation of technology and sample source, it is impossible to complete the cross-reaction test of all related or similar substances. Therefore, this kit may have cross-reaction with other untested substances.

Stability
It has been determined that the kit must be stored at the recommended temperature within the validity period, and the activity reduction rate is less than 5%. In order to reduce the influence of external factors on the detection value before and after the destruction of the kit, the environmental conditions of the laboratory should be kept as consistent as possible, especially the temperature, humidity and incubation conditions in the laboratory. Secondly, the operation by the same experimenter can reduce human error.

Kit composition

Serial number	English name	specification
1	Wash solution(30X)	20ml×1 bottle
2	HRP-Conjugate reagent	6ml×1 bottle
3	Microelisa stripplate	12 well×8 strips
4	Sample diluent	6ml×1 bottle
5	Chromogen Solution A	6ml×1 bottle
6	Chromogen Solution B	6ml×1/bottle
7	Stop solution	6ml×1 bottle
8	Standard	0.5ml×1 bottle
9	Standard diluent	1.5ml×1 bottle
10	Product Description	1 serving
11	Sealing plate membrane	2 sheets
12	Sealing bag	1 piece

Note: Please check whether the label and quantity of the reagents in the kit are consistent with the table before use.

Important notes

1. The kit takes out from the refrigeration environment should be balanced 15-30 minutes in

the room temperature, ELISA plates coated if has not use up after opened, the plate should

be stored in Sealed bag.

2. washing buffer will Crystallization separation, it can be heated the water helps dissolve

when dilute . Washing does not affect the result.

3. add Sample with sampler Each step, And proofread its accuracy frequently, avoids the

experimental error. add sample within 5 min, if the number of sample is much , recommend

to use Volley .

4. if the testing material content is excessively higher (The sample OD is bigger than the first

standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution

factor.（×n×5）.

5. Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6. The substrate evade the light preservation.

7. Please according to use instruction strictly, The test result determination must take the

microtiter plate reader as a standard.

8. All samples, washing buffer and each kind of reject should according to infective material

process.

9. Do not mix reagents with those from other lots.

Storage conditions and validity period
1. Store at 2-8℃, do not freeze, the validity period is 6 months.
2. After opening and use, put the coated microplate into a ziplock bag with desiccant, seal the ziplock bag, and put all reagents back into the refrigerator at 2-8°C.

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Rat Calcitonin（CT）ELISA KIT