Name: Rat Interleukin 6 (IL-6) ELISA Kit instruction
Brand: Biological test kits, ELISA (enzyme-linked immunosorbent assay) kits, chemical reagents-Shanghai IDEAL MEDICAL TECH Co. Ltd.
SKU: ADL01597R1
Availability: InStock
Rating: 5 (1 reviews)

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Home > ELISA Kit > Researchuse ELISA kit > Rat ELISA kit

Rat Interleukin 6 (IL-6) ELISA Kit instruction

Description

Rat Interleukin 6 (IL-6) ELISA Kit instruction
Specifications	48T/96T
article number	ADL01597R1
Species	rat
Sample type	serum, plasma, tissue homogenate,cell lysate, cell Culture supernatant etc.
Detection range	See the manual for details
Sensitivity	See the manual for details
Response time	1-5h
Sample volume	100μL
Detection wavelength	450nm
Delivery date	3-5 days
Manual	Consult our salesperson for obtaining

Intended use

This IL-6 ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of IL-6 in the sample, this IL-6 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus IL-6 concentration. The concentration of IL-6 in the samples is then determined by comparing the O.D. of the samples to the \standard curve.

Recovery rate
The target protein of known concentration was added to 5 different samples, and the recovery experiment was performed to obtain the recovery range and average recovery rate.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	94-110	102
EDTA plasma (n=5)	90-102	96
Cell culture media (n=5)	95-106	98

Linear
Add the target protein of known concentration to 5 samples respectively, do recovery experiment, get the range of recovery rate and average recovery rate. Dilute 5 samples by 2 times, 4 times, 8 times, and 16 times respectively for recovery experiments to obtain the recovery range and average recovery rate.

Sample	1:2	1：4	1:8	1:16
Serum (n=5)	99-108%	95-109%	89-103%	88-102%
EDTA plasma (n=5)	95-105%	90-100%	89-104%	98-108%
Cell culture media (n=5)	92-104%	97-105%	96-108%	92-105%

Specificity
This kit recognizes natural and Rat soluble E-selectin, and has no obvious cros-reactivity with other similar substances. Due to the limitation of technology and sample source, it is impossible to complete the cross-reaction test of all related or similar substances. Therefore, this kit may have cross-reaction with other untested substances.

Stability
It has been determined that the kit must be stored at the recommended temperature within the validity period, and the activity reduction rate is less than 5%. In order to reduce the influence of external factors on the detection value before and after the destruction of the kit, the environmental conditions of the laboratory should be kept as consistent as possible, especially the temperature, humidity and incubation conditions in the laboratory. Secondly, the operation by the same experimenter can reduce human error.

Kit composition

Serial number	English name	specification
1	Wash solution(30X)	20ml×1 bottle
2	HRP-Conjugate reagent	6ml×1 bottle
3	Microelisa stripplate	12 holes×8 strips
4	Sample diluent	6ml×1 bottle
5	Chromogen Solution A	6ml×1 bottle
6	Chromogen Solution B	6ml×1/bottle
7	Stop solution	6ml×1 bottle
8	Standard	0.5ml×1 bottle
9	Standard diluent	1.5ml×1 bottle
10	Product Description	1 serving
11	Sealing plate membrane	2 sheets
12	Sealing bag	1 piece

Note: Please check whether the label and quantity of the reagents in the kit are consistent with the table before use.

Materials required but not supplied

1. Standard microplate reader(450nm)

2. Precision pipettes and Disposable pipette tips.

3. 37 ℃ incubator

Storage conditions and validity period
1. Store at 2-8℃, do not freeze, the validity period is 6 months.
2. After opening and use, put the coated microplate into a ziplock bag with desiccant, seal the ziplock bag, and put all reagents back into the refrigerator at 2-8°C.

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Rat Interleukin 6 (IL-6) ELISA Kit instruction