Name: Urease(UE) Assay Kit
Measurement method: Spectrophotometric method
Specification: 50 tubes/24 samples
Storage condition: low temperature and keep away from light
Validity: 6 months
Note: Choose 2-3 samples with large expected differences for pre-calibration before formal determination.
Measurement significance:
UE is able to hydrolyse urea to produce ammonia and carbonic acid.UE activity is positively correlated with organic matter content, total nitrogen and quick nitrogen content, which reflects the nitrogen status.
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Principle of Determination:
Determination of NH3-N produced by urease hydrolysis of urea using the indophenol blue colourimetric method.
Instruments and supplies to be provided:
Visible spectrophotometer, water bath, adjustable pipette, 1ml cuvette, mortar, ice, and distilled water.

